Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114138_7rg5 GGGGGGTAGCCTGGACTGGCAGGTGGGCAGAG(+AAAAAA)GTACCTGTGTGGCCAGGGCCACTCTAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606114136_lfoc ACTTACAGACTAGAGTGGCCCTGGCCACACAG(+AAAAAA)GTACCTCTGCCCACCTGCCAGTCCAGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240606114135_ulnu AGGGAGAGGAGGAAGAGGGCAGCATCCAGAGG(+AAAAAA)GATCCCTATGGACCCCAGCACTGACTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606114133_2dcb TGGGTGCTGCAGTCAGTGCTGGGGTCCATAGG(+AAAAAA)GATCCCTCTGGATGCTGCCCTCTTCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606114131_j1ev CTTGATCCCAGGTGGATTCCCACCCTAACTGG(+AAAAAA)GTACCAGGACTCTGAACAGGCCAAGATGGGG NGG 10 8 17 8 24 0 100 5 10
    240606114130_icxt GAGTGCCCCATCTTGGCCTGTTCAGAGTCCTG(+AAAAAA)GTACCCAGTTAGGGTGGGAATCCACCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606114127_2bs4 GTGTCTCTTGGCACTGTCACCAAGTTGCCCAG(+AAAAAA)GATCCTGGGGATGCTGTAATTTGGAAAATTG NGG 10 8 17 8 24 0 100 5 10
    240606114124_bhq2 GCCCTCAATTTTCCAAATTACAGCATCCCCAG(+AAAAAA)GATCCTGGGCAACTTGGTGACAGTGCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606114123_pf4i CAGCAGCCACTGGGAAGAAGAGGCACAACAAG(+AAAAAA)GATCCATCCTCCCAGAGACCTTTCCAACTCC NGG 10 8 17 8 24 0 100 5 10
    240606114122_6yma TGGCAGGAGTTGGAAAGGTCTCTGGGAGGATG(+AAAAAA)GATCCTTGTTGTGCCTCTTCTTCCCAGTGGC NGG 10 8 17 8 24 0 100 5 10