Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114047_4kmy TCTCACCTGAGAAATGGTGCAAACCAACCAGG(+AAAAAA)GATCCTTAGGCTACTGAGAAGGCTGTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606114045_j8tq AGGTAAGCAGACAGCCTTCTCAGTAGCCTAAG(+AAAAAA)GATCCCTGGTTGGTTTGCACCATTTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606114043_xm48 GGGCCCTGTGACCTTCAACATCTGGCAGGGGG(+AAAAAA)GATCCTGGGACAGGGGGTGGAGAAGGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240606114040_1e5j GTGTCAACTGCCTTCTCCACCCCCTGTCCCAG(+AAAAAA)GATCCCCCCTGCCAGATGTTGAAGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606114039_i6vv TCACTAAGAGGACTGAAGGATCTAGTTGAGAG(+AAAAAA)GATCCCTCATGAACGTCCACTACCAGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606114037_rhwf TGAGCTCTGGCTGGTAGTGGACGTTCATGAGG(+AAAAAA)GATCCTCTCAACTAGATCCTTCAGTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606114036_jjrx TGGATAGGGTTGGAGCTACTGGGACAGAGCTG(+AAAAAA)GATCCAGCCATGAGTCTTATGTCCTCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606114035_us1f TCTGCTCTGGGAGGACATAAGACTCATGGCTG(+AAAAAA)GATCCAGCTCTGTCCCAGTAGCTCCAACCCT NGG 10 8 17 8 24 0 100 5 10
    240606114033_dvm0 GGATCTTCCAGACGCTGAATCGCACGTAGGTG(+AAAAAA)GTACCTACTGTGCTCCTTGGCTGTGCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606114032_qzv8 TGCTGAGACTGCACAGCCAAGGAGCACAGTAG(+AAAAAA)GTACCACCTACGTGCGATTCAGCGTCTGGAA NGG 10 8 17 8 24 0 100 5 10