Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114013_lz8b CATGGCTTGGGTTGTGGACCTTCTTCCCCATG(+AAAAAA)GATCCAGAGTGGCACAGGGTCCTGCCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606114012_0ns5 CTGTGCTCAGGGCAGGACCCTGTGCCACTCTG(+AAAAAA)GATCCATGGGGAAGAAGGTCCACAACCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606114010_15ck TGACCTGGCCAATGTACAGGCCTTTGTCTCAG(+AAAAAA)GATCCTTGCCTGGTTTGCACAATAGGAAATG NGG 10 8 17 8 24 0 100 5 10
    240606114009_sdnu CACCACATTTCCTATTGTGCAAACCAGGCAAG(+AAAAAA)GATCCTGAGACAAAGGCCTGTACATTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606114006_hmpm CAACACTGCACCACTGAACTGTGTCCCCCAGG(+AAAAAA)GTACCATCACAGAGAATGGACAAAAAATGAG NGG 10 8 17 8 24 0 100 5 10
    240606114005_4r6z GTGGGCTCATTTTTTGTCCATTCTCTGTGATG(+AAAAAA)GTACCCTGGGGGACACAGTTCAGTGGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606114004_v3er TGCTAGTTGCTCTGAAAGATTGGGACCTCTTG(+AAAAAA)GATCCCCCCCCCCATGGACTCTGATTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606114001_aor6 ATAGACAGACAATCAGAGTCCATGGGGGGGGG(+AAAAAA)GATCCAAGAGGTCCCAATCTTTCAGAGCAAC NGG 10 8 17 8 24 0 100 5 10
    240606114000_isi0 AACCCCCGCCAGCCCAGTAGGGTAGCCAGGAG(+AAAAAA)GATCCCTGAGTCCAACCCGTAAACACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240606113958_v8d3 TGTGGCTCTAGTGTTTACGGGTTGGACTCAGG(+AAAAAA)GATCCTCCTGGCTACCCTACTGGGCTGGCGG NGG 10 8 17 8 24 0 100 5 10