Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113957_5qnk GCTATAGAGGGCAAACCTGTTTGTATTCATGG(+AAAAAA)GATCCAACAAAATACATGAAAGGATGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606113955_ggad CATATTGGCTCATCCTTTCATGTATTTTGTTG(+AAAAAA)GATCCCATGAATACAAACAGGTTTGCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606113954_4luw TCACTGAGCTCTGTTCTCTGTCTGCAGCACAG(+AAAAAA)GTACCAAAGAGGGGTGCAATGGAGGGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240606113953_rfcy TCAGAGCTAGCCCTCCATTGCACCCCTCTTTG(+AAAAAA)GTACCTGTGCTGCAGACAGAGAACAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606113951_rdfq AGTCATAGCTTCTCAGCAGTTGTGAAAAGTGG(+AAAAAA)GTACCATAGCCAGCTCCACTAGCTTCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606113950_6j92 CCAATGCCCTGAAGCTAGTGGAGCTGGCTATG(+AAAAAA)GTACCCACTTTTCACAACTGCTGAGAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606113949_33ck TTTGCTGGACCTGATCCCCAAGCACAGGAGTG(+AAAAAA)GTACCCTCCACAGAAGCTGGGGGAGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606113947_vas3 TGCTGCAGACTCTCCCCCAGCTTCTGTGGAGG(+AAAAAA)GTACCACTCCTGTGCTTGGGGATCAGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240606113946_cf5o ACTATTAACTATAATAGAAGTCACTAGGAAGG(+AAAAAA)GTACCATGCTCTCAGAATCCCCACGTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606113945_urrn CGTGGCTGGGACGTGGGGATTCTGAGAGCATG(+AAAAAA)GTACCCTTCCTAGTGACTTCTATTATAGTTA NGG 10 8 17 8 24 0 100 5 10