Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114030_0qsq AAGACCACTTGCTGTTCAAGTTTAAGTCCCAG(+AAAAAA)GATCCACATTGGATGGCTCACAACCGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606114029_gyg5 GTCAGACAAGCGGTTGTGAGCCATCCAATGTG(+AAAAAA)GATCCTGGGACTTAAACTTGAACAGCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606114027_f32d GCAACCAGGACTCTGAGAGGTTGTGCTGACGG(+AAAAAA)GATCCCTATTTATTATTTTCATCTACTTGGT NGG 10 8 17 8 24 0 100 5 10
    240606114025_1wue TTCGTACCAAGTAGATGAAAATAATAAATAGG(+AAAAAA)GATCCCGTCAGCACAACCTCTCAGAGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606114023_1jeu AAATTGCTGAGTGAAGCCATGCAAGACGGAGG(+AAAAAA)GATCCAGGATAGGGTCCAGTCCCTGTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606114021_959c AGCAGACAGGACAGGGACTGGACCCTATCCTG(+AAAAAA)GATCCCTCCGTCTTGCATGGCTTCACTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606114020_0rx2 GGGGGTGAGGAAAGAGAGAAGGGGAGTCGGAG(+AAAAAA)GATCCAGCAGGCCACTCTTTCCACCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240606114019_81u9 GTCCCATGGGAGGTGGAAAGAGTGGCCTGCTG(+AAAAAA)GATCCTCCGACTCCCCTTCTCTCTTTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240606114018_wls9 TGCAGTGGCCACAAAGGCCAGAGAGGCTGTTG(+AAAAAA)GATCCCCTTGAGCTGGAGTTACAGGCGATCG NGG 10 8 17 8 24 0 100 5 10
    240606114016_ke99 GCAAACGATCGCCTGTAACTCCAGCTCAAGGG(+AAAAAA)GATCCAACAGCCTCTCTGGCCTTTGTGGCCA NGG 10 8 17 8 24 0 100 5 10