Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114224_us96 AGAAGTCCCTGACAAGATGGTGTTTGGCTTGG(+AAAAAA)GATCCAGAGATGTCCAAATCCTGTCTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606114223_n1gv GCCACGTGGAAGACAGGATTTGGACATCTCTG(+AAAAAA)GATCCCAAGCCAAACACCATCTTGTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606114222_x7kl CTCCAGACCCATTTTTAGATAAGGGATTCAAG(+AAAAAA)GATCCAAGAAGACTTGTCCTGAATTCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606114221_2p6n GCTAGCTCAGGAATTCAGGACAAGTCTTCTTG(+AAAAAA)GATCCTTGAATCCCTTATCTAAAAATGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606114219_g3c8 TGACCATTTCTGTAATTGGTACTGTCTGGCAG(+AAAAAA)GTACCATGTGTGGCCTGGCTGTCCTGGAACA NGG 10 8 17 8 24 0 100 5 10
    240606114218_ebl1 TGCTGTGTTCCAGGACAGCCAGGCCACACATG(+AAAAAA)GTACCTGCCAGACAGTACCAATTACAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240606114216_xuor GGATGGACACAGGTCCCCCAGGGCCTAGGATG(+AAAAAA)GTACCTTGAGAAGACCAGCCTCATCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240606114215_w87l GGGCCATGGATGATGAGGCTGGTCTTCTCAAG(+AAAAAA)GTACCATCCTAGGCCCTGGGGGACCTGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606114214_zhk9 AACTCAATTCTTGTTGGAAAAACAACAAAAAG(+AAAAAA)GTACCTAATCTAGTACAGCACCCTCAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606114213_6zfq CCTGGCTGGCTGAGGGTGCTGTACTAGATTAG(+AAAAAA)GTACCTTTTTGTTGTTTTTCCAACAAGAATT NGG 10 8 17 8 24 0 100 5 10