Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114335_vb5p AAACAGCTTCATGTTTGCCCCATTTCTGTCAG(+AAAAAA)GATCCCCCAACCATGGGGGGCACTTGCACAA NGG 10 8 17 8 24 0 100 5 10
    240606114332_30r7 TATTTTTGTGCAAGTGCCCCCCATGGTTGGGG(+AAAAAA)GATCCTGACAGAAATGGGGCAAACATGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606114331_hapn GCCCTTAGCATGACGGGTTCTGCCCTTTGTGG(+AAAAAA)GATCCGGTAAGGAAACTGAGAGGTTCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606114329_fb3g TTGAAACAAGGAACCTCTCAGTTTCCTTACCG(+AAAAAA)GATCCCACAAAGGGCAGAACCCGTCATGCTA NGG 10 8 17 8 24 0 100 5 10
    240606114327_elbu ATTATAGGACAGAAAGTGCTGGGGTTGGGGAG(+AAAAAA)GTACCAAGAGGGTGAGTAGTAGGGGGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240606114326_tdd9 GGGGAGTCTTCCCCCTACTACTCACCCTCTTG(+AAAAAA)GTACCTCCCCAACCCCAGCACTTTCTGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606114324_uxb0 ATTGTTCAGTTCTGTGTGTGAGGGTAGGCAGG(+AAAAAA)GTACCTGCCCCATGTCAGCCGCCCTGATGAG NGG 10 8 17 8 24 0 100 5 10
    240606114322_z02b TTCTTCTCATCAGGGCGGCTGACATGGGGCAG(+AAAAAA)GTACCCTGCCTACCCTCACACACAGAACTGA NGG 10 8 17 8 24 0 100 5 10
    240606114321_mf52 TTACCCCAATTGTCATAAGGCCTGAGAGAATG(+AAAAAA)GTACCCACTTTCCCTGTCCCAAAGTGCCTCA NGG 10 8 17 8 24 0 100 5 10
    240606114320_frjx CTTTTTGAGGCACTTTGGGACAGGGAAAGTGG(+AAAAAA)GTACCATTCTCTCAGGCCTTATGACAATTGG NGG 10 8 17 8 24 0 100 5 10