Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113103_zr4k AAGGGCACTTGGCTCAGTTGGAGACACTTTTG(+AAAAAA)GTACCTGGTTGGGCAAGTGGGGTGGTACTAA NGG 10 8 17 8 24 0 100 5 10
    240606113101_7cix CGGTATTAGTACCACCCCACTTGCCCAACCAG(+AAAAAA)GTACCAAAAGTGTCTCCAACTGAGCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606113100_7h83 AAGTGGATTGAGTTTTTAGCACCTGAGTGTGG(+AAAAAA)GATCCATTGTCATTTGTTTTCCCAGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240606113058_s6rk AAGTAAAGGTTCTGGGAAAACAAATGACAATG(+AAAAAA)GATCCCACACTCAGGTGCTAAAAACTCAATC NGG 10 8 17 8 24 0 100 5 10
    240606113056_km59 GGGGATCTGATGCCCTCTTCTGGCCTCGGTGG(+AAAAAA)GTACCACAAGTGCATCCGATGCCCAGGCATC NGG 10 8 17 8 24 0 100 5 10
    240606113055_bobm GCACAGATGCCTGGGCATCGGATGCACTTGTG(+AAAAAA)GTACCCACCGAGGCCAGAAGAGGGCATCAGA NGG 10 8 17 8 24 0 100 5 10
    240606113054_vrp4 CCCCTGTTCTGTTCTTCGCGGCCTTGCCTGAG(+AAAAAA)GATCCGTGTTCTGTGTGAACCATGAGAATTC NGG 10 8 17 8 24 0 100 5 10
    240606113052_m5y5 ATGAGGAATTCTCATGGTTCACACAGAACACG(+AAAAAA)GATCCTCAGGCAAGGCCGCGAAGAACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606113049_qlsu TTCTGGCAGACCAGACGACCAGTTAGCCCTGG(+AAAAAA)GATCCGTTTCTGTGCCCTGCATCCCGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606113048_n77w CTGTGGGGAACGGGATGCAGGGCACAGAAACG(+AAAAAA)GATCCCAGGGCTAACTGGTCGTCTGGTCTGC NGG 10 8 17 8 24 0 100 5 10