Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113046_2iro GAAGTACCTGCAATATCAGCTCACTAACACGG(+AAAAAA)GTACCACTAGTTCTTGTTTCTCTTTGGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606113045_ipfp AAAATGACACCAAAGAGAAACAAGAACTAGTG(+AAAAAA)GTACCCGTGTTAGTGAGCTGATATTGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606113042_lzvj ACTGCAACATGAGTTGGAGGGAAGAGCCCTGG(+AAAAAA)GTACCTGCCTGGTCCCCTCTGCTTCTATGAA NGG 10 8 17 8 24 0 100 5 10
    240606113041_gl1j TGGAGTTCATAGAAGCAGAGGGGACCAGGCAG(+AAAAAA)GTACCCAGGGCTCTTCCCTCCAACTCATGTT NGG 10 8 17 8 24 0 100 5 10
    240606113039_qvh3 CATATGGGCTATGCAGAGAGATAGGACTTAGG(+AAAAAA)GTACCAGAACTTCCTGCTCTGTGGTCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606113038_lc26 GGATACCTGGGACCACAGAGCAGGAAGTTCTG(+AAAAAA)GTACCCTAAGTCCTATCTCTCTGCATAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606113036_0hme TTCCCCCTACACTCATTCTGCTTCCCCAGAAG(+AAAAAA)GATCCTAACACAGAAGATCCCACTCTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606113035_tn63 TTACGGTGCTAGAGTGGGATCTTCTGTGTTAG(+AAAAAA)GATCCTTCTGGGGAAGCAGAATGAGTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240606113033_7y8g TATTGAATTTCTTCAGCAGCCAATGAATCCTG(+AAAAAA)GATCCATACAAAAATATATTTAGCTTCCACC NGG 10 8 17 8 24 0 100 5 10
    240606113032_o7ce TTGCAGGTGGAAGCTAAATATATTTTTGTATG(+AAAAAA)GATCCAGGATTCATTGGCTGCTGAAGAAATT NGG 10 8 17 8 24 0 100 5 10