Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112404_sb7w CCTAGACCCTCCAATGCCCAGTCGCTGGCATG(+AAAAAA)GATCCAGGCATACACACACATGCACACACAT NGG 10 8 17 8 24 0 100 5 10
    240606112402_enwl GACTTAGGGTATAGGACATACGCCATGACAGG(+AAAAAA)GATCCTATAAGGAAAACGTGCTAGACATCAG NGG 10 8 17 8 24 0 100 5 10
    240606112401_jwe7 GTTTGCTGATGTCTAGCACGTTTTCCTTATAG(+AAAAAA)GATCCCTGTCATGGCGTATGTCCTATACCCT NGG 10 8 17 8 24 0 100 5 10
    240606112400_kynp CATCTGTGCTCCATTGCTCCAAAAGCCAGCAG(+AAAAAA)GTACCTGAGGGCTAGTGTCTGAACTCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606112358_q3ke TGAAAGCTGAGAGTTCAGACACTAGCCCTCAG(+AAAAAA)GTACCTGCTGGCTTTTGGAGCAATGGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606112356_teec GAGGAACCTTCAACGTAGGCAGCTATTAAATG(+AAAAAA)GATCCAGTATTTCTCAGAGCATCCTATGCAA NGG 10 8 17 8 24 0 100 5 10
    240606112355_do6r TCACCTTGCATAGGATGCTCTGAGAAATACTG(+AAAAAA)GATCCATTTAATAGCTGCCTACGTTGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606112354_tb8i TGAGTGACCTGGGATACATAGACACCCTTATG(+AAAAAA)GATCCTTGACATCTGTGCTCCATTGCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606112353_h9vh GGCTTTTGGAGCAATGGAGCACAGATGTCAAG(+AAAAAA)GATCCATAAGGGTGTCTATGTATCCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606112351_6hmt TCCCTGGCTCCACTATCGAACTGCTGGGTGGG(+AAAAAA)GATCCGTTCCTGCTATTACCCTCTTCTACTG NGG 10 8 17 8 24 0 100 5 10