Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112450_wwu8 CGGGCCCTCCTGGAGATGCCTGTGGCTAGTGG(+AAAAAA)GATCCGGAGAGCACAGGGTGGGGCGGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606112449_byh2 GCACCCAGATAGGCAAGGTCACCTTGACACTG(+AAAAAA)GTACCATGGTAACCTTGGCTCTTTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606112448_q5ce TGCACCAGCCCTAAAGAGCCAAGGTTACCATG(+AAAAAA)GTACCAGTGTCAAGGTGACCTTGCCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240606112446_mzr0 CTGTGCTACCACACCCAGCCTGAGTTTAAATG(+AAAAAA)GTACCCTCGGACCCAAGGCAGCTCTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240606112444_khvd TGTCTTAGCACAGAGCTGCCTTGGGTCCGAGG(+AAAAAA)GTACCATTTAAACTCAGGCTGGGTGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606112443_jwyt CTGTGCTACCACACCCAGCCTGAGTTTAAATG(+AAAAAA)GTACCCTCGGACCCAAGGCAGCTCTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240606112441_i79j TGTCTTAGCACAGAGCTGCCTTGGGTCCGAGG(+AAAAAA)GTACCATTTAAACTCAGGCTGGGTGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240606112439_p6f4 CTTCATTGGCATGGCAACAGGTAGGCATATGG(+AAAAAA)GATCCTCCTAGTGGTTAGTACAATCACACCA NGG 10 8 17 8 24 0 100 5 10
    240606112438_tltv CACAGTGGTGTGATTGTACTAACCACTAGGAG(+AAAAAA)GATCCCATATGCCTACCTGTTGCCATGCCAA NGG 10 8 17 8 24 0 100 5 10
    240606112436_ba9b ACATGGACCTTTGGAAACCTGTGGATGACATG(+AAAAAA)GATCCGTGAAGAGCCCGGGTCACTACCACTC NGG 10 8 17 8 24 0 100 5 10