Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112537_xkzg TATTGGAAGGGTCATCTCAGTCTTTGGTCCGG(+AAAAAA)GATCCTGGGCCTGATGAGCCCATAGTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606112536_3vr4 ATGCATGCTCTCTGTCTTTTGCTACAAAAATG(+AAAAAA)GTACCCAGATCTGCCTACTGCTTTCCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606112534_m58j GGGGAGGGCAGGAAAGCAGTAGGCAGATCTGG(+AAAAAA)GTACCATTTTTGTAGCAAAAGACAGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606112533_tsn5 ATGCATGCTCTCTGTCTTTTGCTACAAAAATG(+AAAAAA)GTACCCAGATCTGCCTACTGCTTTCCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606112532_cqt5 GGGGAGGGCAGGAAAGCAGTAGGCAGATCTGG(+AAAAAA)GTACCATTTTTGTAGCAAAAGACAGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606112529_67gy CAAGAGATTAAACTCCTGGCCAGAGAGTGGGG(+AAAAAA)GTACCATATGGGAAAGGGGGAGCTAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606112528_8z0p ATGCCCTGGCTTAGCTCCCCCTTTCCCATATG(+AAAAAA)GTACCCCCACTCTCTGGCCAGGAGTTTAATC NGG 10 8 17 8 24 0 100 5 10
    240606112527_iwjh GAGCTGACACGGTTCAAGGCCTGTGTGTCTAG(+AAAAAA)GTACCCCGAGGCGATGCCGAGGGCTGCCACC NGG 10 8 17 8 24 0 100 5 10
    240606112525_e11t TGGTTGGTGGCAGCCCTCGGCATCGCCTCGGG(+AAAAAA)GTACCTAGACACACAGGCCTTGAACCGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606112524_f218 GGTGCTTCCTTACTCCAGTTACTTCCTGCTGG(+AAAAAA)GTACCAGTTTCTCTCAGAGGAGGACGCTGTC NGG 10 8 17 8 24 0 100 5 10