Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112556_yc87 AGCTGGTGGCAGCCTGAGTGTCCCTTGGTCAG(+AAAAAA)GATCCCAGCTCTGATGCCTGTCAGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606112554_v9t3 ACAGATAGTGTATGAAATACACAATTTCCCAG(+AAAAAA)GTACCAAGGGCCAATAGCAAAAAGGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606112551_qjw8 CTTTTAGAGACCCTTTTTGCTATTGGCCCTTG(+AAAAAA)GTACCTGGGAAATTGTGTATTTCATACACTA NGG 10 8 17 8 24 0 100 5 10
    240606112548_v51s TTGACTGTCTCTGCATAGTCTTGATACCCCAG(+AAAAAA)GATCCTGACATCCCCAGAAATTATACCCCGC NGG 10 8 17 8 24 0 100 5 10
    240606112547_lsgc GGGGGGCGGGGTATAATTTCTGGGGATGTCAG(+AAAAAA)GATCCTGGGGTATCAAGACTATGCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240606112546_uoam TGCACACAGTGAGGAATAAACATTCTGGAAAG(+AAAAAA)GATCCTTGATTCTCCCACAAACTGTTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606112545_3uyu TACTCCAAGGAACAGTTTGTGGGAGAATCAAG(+AAAAAA)GATCCTTTCCAGAATGTTTATTCCTCACTGT NGG 10 8 17 8 24 0 100 5 10
    240606112543_1ks1 TTTCCTTTTTAAATCATCCTGTGTGCATTCAG(+AAAAAA)GATCCTGGTCTTCATACAGAGATGTTGACAC NGG 10 8 17 8 24 0 100 5 10
    240606112542_o3is GAAGGGTGTCAACATCTCTGTATGAAGACCAG(+AAAAAA)GATCCTGAATGCACACAGGATGATTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606112539_fni7 TTTGGTCTGGACTATGGGCTCATCAGGCCCAG(+AAAAAA)GATCCCGGACCAAAGACTGAGATGACCCTTC NGG 10 8 17 8 24 0 100 5 10