Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112653_co8i ATCCTGCCATGTGGCTGGTTACCTGTGCTCAG(+AAAAAA)GTACCATCCGTCCATCTCCTCACATCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606112651_up5e ACCCAGGGAAGATGTGAGGAGATGGACGGATG(+AAAAAA)GTACCTGAGCACAGGTAACCAGCCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240606112650_ioxh AAAACCCACAAACCTGACAGCGGGCAGAGGAG(+AAAAAA)GATCCAGAACTATTGCTGATACCAAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606112649_fdgm AGAAGCAGCTCTTGGTATCAGCAATAGTTCTG(+AAAAAA)GATCCTCCTCTGCCCGCTGTCAGGTTTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606112648_2b4w TCTACTTCAGAGGCGATGAGGATAACCTGCAG(+AAAAAA)GATCCTAAGCATGTGCAACAGGCCATGAACC NGG 10 8 17 8 24 0 100 5 10
    240606112646_3h7b CCAAAGGTTCATGGCCTGTTGCACATGCTTAG(+AAAAAA)GATCCTGCAGGTTATCCTCATCGCCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606112645_k6rx GCCCCTCATCTCTATCGTTGCTTTTGTTCAAG(+AAAAAA)GATCCAGTCAAGGCTAACATGTTCCGCGGCC NGG 10 8 17 8 24 0 100 5 10
    240606112644_qin5 GATGTGGCCGCGGAACATGTTAGCCTTGACTG(+AAAAAA)GATCCTTGAACAAAAGCAACGATAGAGATGA NGG 10 8 17 8 24 0 100 5 10
    240606112643_sayv GATGCCCCCCCCCCAACCCAGTGTATTCTCTG(+AAAAAA)GATCCCTAAGTGCCTTAGATCCTGAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240606112641_b399 AGCTCTGTGGCTCAGGATCTAAGGCACTTAGG(+AAAAAA)GATCCAGAGAATACACTGGGTTGGGGGGGGG NGG 10 8 17 8 24 0 100 5 10