Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112722_z238 ACATCTCCTGCCTCAGTCTTCAAAGCTATTTG(+AAAAAA)GATCCATAAGTTTGTTATATGCATCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240606112720_mov4 ACATGCAAGAAGATGCATATAACAAACTTATG(+AAAAAA)GATCCAAATAGCTTTGAAGACTGAGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606112719_7ekn CAAGGTAATGAGTTTCGTGGTAGCATTTTGAG(+AAAAAA)GATCCATGTCATCGTTCATTGTTCTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606112718_tt0b GGGCTAGGAAAAGAACAATGAACGATGACATG(+AAAAAA)GATCCTCAAAATGCTACCACGAAACTCATTA NGG 10 8 17 8 24 0 100 5 10
    240606112717_dua3 CTTGCAAAAGCCAAAGCTGCCTGAGTAGTCTG(+AAAAAA)GATCCTGCAACATCACTTTGCTTCAGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606112716_45va AAACTAACTCCTGAAGCAAAGTGATGTTGCAG(+AAAAAA)GATCCAGACTACTCAGGCAGCTTTGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240606112714_3hzc CTCCAAAGCTAGAGTAATCATGAGGGAGGCAG(+AAAAAA)GATCCACGGAGAATCACAAAGGATGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240606112712_n34a GTATCAAAATGCATCCTTTGTGATTCTCCGTG(+AAAAAA)GATCCTGCCTCCCTCATGATTACTCTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606112710_pqdc GTATGCTGAGGTGTGGGGGTGGTGAATGAAGG(+AAAAAA)GTACCCTACCTGCAGTGCTCAAGAATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240606112709_ybtr ATCATTGGACATTCTTGAGCACTGCAGGTAGG(+AAAAAA)GTACCCTTCATTCACCACCCCCACACCTCAG NGG 10 8 17 8 24 0 100 5 10