Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114454_3ozv GCTAAAAATTCTGCCTCTCTGTCACTGTGCTG(+AAAAAA)GATCCGTCTAAAGTGGGACAGTTCAATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606114452_fffe TTTCAGACCATTGAACTGTCCCACTTTAGACG(+AAAAAA)GATCCAGCACAGTGACAGAGAGGCAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240606114451_xylz ACCCAGTCTAAGCCCAGAGCTTGTTTGGGAGG(+AAAAAA)GATCCTCTCCTTCCTCCTGCTTGATCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606114449_z6by GCTCCTACAGGATCAAGCAGGAGGAAGGAGAG(+AAAAAA)GATCCCTCCCAAACAAGCTCTGGGCTTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606114447_2zdi CTTGATGTGTGAGGGGTAATACCCAGAGTGGG(+AAAAAA)GTACCTGCTTCACAAAGGATAAGGGGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240606114446_k19g ATTCCACCCTCCCCTTATCCTTTGTGAAGCAG(+AAAAAA)GTACCCCACTCTGGGTATTACCCCTCACACA NGG 10 8 17 8 24 0 100 5 10
    240606114444_u5mp TGCCTGCCTGCCTGCCTGCCTGCCTACCTGTG(+AAAAAA)GATCCTGTTCCCCTAAATGGTCTGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240606114443_k0v6 GGCCAGACAAGGCAGACCATTTAGGGGAACAG(+AAAAAA)GATCCACAGGTAGGCAGGCAGGCAGGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606114441_tscx AGGAAGGCAGGAGGGGTGCCCGTCCCGTTGGG(+AAAAAA)GATCCTAGCACAAGGCCTGACCAGGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606114440_xf4p AAGTCCAGGGACCTGGTCAGGCCTTGTGCTAG(+AAAAAA)GATCCCCAACGGGACGGGCACCCCTCCTGCC NGG 10 8 17 8 24 0 100 5 10