Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112640_j5cr GGGTCCAGGCTCTTCAGGCTCAGTAGGGTCAG(+AAAAAA)GTACCAGCTATACTCTGGTTGTTAAAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606112639_4948 ACCCCAAGCCTTTAACAACCAGAGTATAGCTG(+AAAAAA)GTACCTGACCCTACTGAGCCTGAAGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606112637_16a4 ATAACTGGCTGGGGGGTTAGGCAAAGGACCTG(+AAAAAA)GTACCCTTACTACTCAAAGCCTACCTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606112635_rq2b ACTGCCCACTAGGTAGGCTTTGAGTAGTAAGG(+AAAAAA)GTACCAGGTCCTTTGCCTAACCCCCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606112634_noch ACCACGGCCTCTGAATGGGGCTGAGGCTCATG(+AAAAAA)GATCCCGGAATGAGACCAGGATGCATAAGAC NGG 10 8 17 8 24 0 100 5 10
    240606112632_yetz CACTGGTCTTATGCATCCTGGTCTCATTCCGG(+AAAAAA)GATCCATGAGCCTCAGCCCCATTCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606112631_0x09 GGCTTCTTGTGATCTCCTTTGCCAGTCACTGG(+AAAAAA)GATCCAGAACAATCTACTTCTTCAAGCATGC NGG 10 8 17 8 24 0 100 5 10
    240606112630_yghz CAATGGCATGCTTGAAGAAGTAGATTGTTCTG(+AAAAAA)GATCCCAGTGACTGGCAAAGGAGATCACAAG NGG 10 8 17 8 24 0 100 5 10
    240606112628_jyo7 GGGTGACTGGATAACCCCAGCATGCCCAGCGG(+AAAAAA)GTACCATAAGCCCACACTGCTGGGATGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606112627_80wj CTGGGACCTCATCCCAGCAGTGTGGGCTTATG(+AAAAAA)GTACCCGCTGGGCATGCTGGGGTTATCCAGT NGG 10 8 17 8 24 0 100 5 10