Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112350_0jru CATCTCAGTAGAAGAGGGTAATAGCAGGAACG(+AAAAAA)GATCCCCACCCAGCAGTTCGATAGTGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606112349_qy0f GACCCTTGGGTGTTCAGAACTCCTGCCAGGAG(+AAAAAA)GATCCTCAGTGCCCACTAAGTCCATGCCATC NGG 10 8 17 8 24 0 100 5 10
    240606112347_p3tc CTGGGGATGGCATGGACTTAGTGGGCACTGAG(+AAAAAA)GATCCTCCTGGCAGGAGTTCTGAACACCCAA NGG 10 8 17 8 24 0 100 5 10
    240606112346_obu2 TTCAATAAATACTTGTTGCATGAACAAACTTG(+AAAAAA)GATCCAAATTTTTAGAAATATTGAATTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606112345_x0kq GAGCATCCAAATTCAATATTTCTAAAAATTTG(+AAAAAA)GATCCAAGTTTGTTCATGCAACAAGTATTTA NGG 10 8 17 8 24 0 100 5 10
    240606112343_ns7u GTGTCCATGTTTAAAGACATCATCCCTGCCAG(+AAAAAA)GATCCATGAGAAAAAATATATTTTCTTACTC NGG 10 8 17 8 24 0 100 5 10
    240606112342_4wbb TGTGGGAGTAAGAAAATATATTTTTTCTCATG(+AAAAAA)GATCCTGGCAGGGATGATGTCTTTAAACATG NGG 10 8 17 8 24 0 100 5 10
    240606112341_n776 AGAGCAGCCTCAGAGAGGCTTGTCCTAGTCAG(+AAAAAA)GATCCAGGATCTTGGCTGGGGCCTGCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606112339_pju8 GAACAAAGAAGCAGGCCCCAGCCAAGATCCTG(+AAAAAA)GATCCTGACTAGGACAAGCCTCTCTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606112338_bar4 CCAGGCATGCTCCTGCCTAGGTGAAATGAAAG(+AAAAAA)GATCCACATACTGGCACATGACATGATCCTA NGG 10 8 17 8 24 0 100 5 10