Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112246_k7uu ACTACAACAACAGTTTAGTAAGAGAAACATGG(+AAAAAA)GTACCTATAGAGTCCTACCATTCATTGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606112245_to14 TAAATCCTGTCTACAAAAATTATGTCATACAG(+AAAAAA)GTACCATTTGTTCTTTCTCTTTCCAAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606112244_eptb AATGGACTCTTTGGAAAGAGAAAGAACAAATG(+AAAAAA)GTACCTGTATGACATAATTTTTGTAGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606112243_roic GAGACGAGTTTCTGAAAATATACTGCACAATG(+AAAAAA)GATCCTAGGAGAGCCCAATCAAAGGCCTACT NGG 10 8 17 8 24 0 100 5 10
    240606112241_dnea AAGAGAGTAGGCCTTTGATTGGGCTCTCCTAG(+AAAAAA)GATCCATTGTGCAGTATATTTTCAGAAACTC NGG 10 8 17 8 24 0 100 5 10
    240606112240_smfl TGGTGTACCAGGCACTATTATTCTTACAGAGG(+AAAAAA)GTACCATGAAAGTTAGATCCAGGTGCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606112238_sb1d GGAGCCAAAGGCACCTGGATCTAACTTTCATG(+AAAAAA)GTACCCTCTGTAAGAATAATAGTGCCTGGTA NGG 10 8 17 8 24 0 100 5 10
    240606112237_pocg CACTGCAAGGTAGTATTTACCTGATCACAAAG(+AAAAAA)GATCCTTTGAGGCTCTACCACATATTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606112236_o2n5 CTAGAATTTGAATATGTGGTAGAGCCTCAAAG(+AAAAAA)GATCCTTTGTGATCAGGTAAATACTACCTTG NGG 10 8 17 8 24 0 100 5 10
    240606112235_bw0e GGCTTAGTTTTTATTTAATAAGATATTTAATG(+AAAAAA)GATCCATTTTTATCAAGGTCAGAAAACATTT NGG 10 8 17 8 24 0 100 5 10