Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112152_hsib CCAACCAGGTTGAGTACTGGGAACCACACTAG(+AAAAAA)GATCCACTGCACCAGCAGAGCACACTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606112151_x7m2 AGAGACAAAGGACACAGGAACCCGGCTGACTG(+AAAAAA)GTACCAAAGTGCACTCTCTTCAGGTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606112150_uwx4 TGTGTAGAGGCACCTGAAGAGAGTGCACTTTG(+AAAAAA)GTACCAGTCAGCCGGGTTCCTGTGTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606112148_p78b TGTCTTTATTCTAATGTCTTTGGTGTGGGAAG(+AAAAAA)GTACCTCATAGGCTGCCAAAAGAGAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240606112147_6b1b ATGTTTGCATTTCTCTTTTGGCAGCCTATGAG(+AAAAAA)GTACCTTCCCACACCAAAGACATTAGAATAA NGG 10 8 17 8 24 0 100 5 10
    240606112146_98bh TGGGTATGGATGGAGCCACACTCCTATGCAAG(+AAAAAA)GATCCATTTCCATGCAAGATTGTCAATTCTT NGG 10 8 17 8 24 0 100 5 10
    240606112145_1iua TCTGGAAGAATTGACAATCTTGCATGGAAATG(+AAAAAA)GATCCTTGCATAGGAGTGTGGCTCCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240606112144_y1an TTTTTAAGATGCTTCTGGAGATTTAGAATCAG(+AAAAAA)GATCCAAAATAAGGCTGGGATTCAGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240606112142_82wh AGTGGTGGATGCTGAATCCCAGCCTTATTTTG(+AAAAAA)GATCCTGATTCTAAATCTCCAGAAGCATCTT NGG 10 8 17 8 24 0 100 5 10
    240606112140_s7x3 TGAGCGTATCTGCTGTCCTTCTGCATGCCCAG(+AAAAAA)GTACCTCAGTTTTTTTCAACAGCAAGGAAAC NGG 10 8 17 8 24 0 100 5 10