Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112108_8s1p GAACTGAAGTTAGACTTGGGAAAGCAATTCTG(+AAAAAA)GATCCACTTAGGATTAGAGTCTACAGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606112107_a5gm AACCTAGAAACTGTAGGTGGGCATATATCAGG(+AAAAAA)GTACCTATGCCACCGCCAACTTCTCCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606112105_u9sd CTTGGACAAAGGAGAAGTTGGCGGTGGCATAG(+AAAAAA)GTACCCTGATATATGCCCACCTACAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606112104_m97b AATGTACTAAATGATTCTCCTCCTCTCTCCTG(+AAAAAA)GTACCTGGAGACCCCTGGCTATCATGGGATT NGG 10 8 17 8 24 0 100 5 10
    240606112102_rbpr TCAGTAATCCCATGATAGCCAGGGGTCTCCAG(+AAAAAA)GTACCAGGAGAGAGGAGGAGAATCATTTAGT NGG 10 8 17 8 24 0 100 5 10
    240606112101_5j71 ACTCAGAAAAGAACCATAGGCACACACGTGTG(+AAAAAA)GATCCTATATCTTGATATTATATAGATACAC NGG 10 8 17 8 24 0 100 5 10
    240606112059_3vth GGATGGTGTATCTATATAATATCAAGATATAG(+AAAAAA)GATCCACACGTGTGTGCCTATGGTTCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606112058_j6wd GGCTATTCCTTGTCCCTACACTGCTCTCTTGG(+AAAAAA)GTACCTCTGGGGCACCCTGGCAGGAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240606112056_ikgz AAAGTTCTCATTCCTGCCAGGGTGCCCCAGAG(+AAAAAA)GTACCCAAGAGAGCAGTGTAGGGACAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606112054_qesu GTGGTACAGGGTCCTGGTCTGCTGTGTGTGGG(+AAAAAA)GATCCTGGCTGCAGAGCTTCTTCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10