Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113821_4sz8 ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+AAAAAA)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606113819_qy83 GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+AAAAAA)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240606113818_emx9 TGGATTCGTATGGCTAACGTCAGTGAATCAAG(+AAAAAA)GATCCCAGGGGATGCCCATTGCTGGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240606113815_j0pn AACTGAGATGCCCAGCAATGGGCATCCCCTGG(+AAAAAA)GATCCTTGATTCACTGACGTTAGCCATACGA NGG 10 8 17 8 24 0 100 5 10
    240606113813_wg8i ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+AAAAAA)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606113812_znba GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+AAAAAA)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240606113811_ghdn TCTACATTTTAATTTTTATGATTCCTGATGTG(+AAAAAA)GATCCATTACAAACTTCTCAGAAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606113810_v8i9 ATGCACTCCAAACTTCTGAGAAGTTTGTAATG(+AAAAAA)GATCCACATCAGGAATCATAAAAATTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606113807_pzsh GGAAGAAAGGTGGTGTCCTTCCGGACTCCGGG(+AAAAAA)GATCCAGGCCTGAATATGGGAGGCACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606113805_5r1m CTGAGCCTGGGTGCCTCCCATATTCAGGCCTG(+AAAAAA)GATCCCCGGAGTCCGGAAGGACACCACCTTT NGG 10 8 17 8 24 0 100 5 10