Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112052_cwp2 TACCTTCTACTCAGAAGAAGCTCTGCAGCCAG(+AAAAAA)GATCCCCACACACAGCAGACCAGGACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606112051_styw CAGCAGTTTTAAAGTCTAACACATTTTAAACG(+AAAAAA)GATCCAAAGTCTTCTTTCCTCATCCGTCACT NGG 10 8 17 8 24 0 100 5 10
    240606112050_cuxr TGCTAAGTGACGGATGAGGAAAGAAGACTTTG(+AAAAAA)GATCCGTTTAAAATGTGTTAGACTTTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606112048_vfzc GGCACTGGAGGAAACTATTTCTCCCTCTCAAG(+AAAAAA)GATCCGGGCGTGCTCTGCACCCGGCCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240606112046_bvc4 AGGCCACCAAGGCCGGGTGCAGAGCACGCCCG(+AAAAAA)GATCCTTGAGAGGGAGAAATAGTTTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606112044_bf73 TGACAGGCTCTGGAGCTGCCTCCTAGGCTGGG(+AAAAAA)GTACCCCTAGGCCCTGGCAACCCACGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606112042_mk5a TGTAAAACAACGTGGGTTGCCAGGGCCTAGGG(+AAAAAA)GTACCCCAGCCTAGGAGGCAGCTCCAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606112041_ht60 TGGTCCTATTCTAGAGAAGAAAGCCTTGCTGG(+AAAAAA)GTACCAATTATGGACTATGGGAGGTAGGATG NGG 10 8 17 8 24 0 100 5 10
    240606112040_fryk AGGAGCATCCTACCTCCCATAGTCCATAATTG(+AAAAAA)GTACCCAGCAAGGCTTTCTTCTCTAGAATAG NGG 10 8 17 8 24 0 100 5 10
    240606112038_8usx GGCTACATGCACGGGCTTCTCTGAGATTCCAG(+AAAAAA)GTACCAGTTCCTTCCGGGTTATGTAATATTA NGG 10 8 17 8 24 0 100 5 10