Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111949_u1az TATGGCTTTGCATGTAAAACACTTTTGATTGG(+AAAAAA)GATCCACAGCAGTGTTCTTTTCACCTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606111947_gqfm CCAGGCCAGCACAGTGCTTCCTGGTAGGGTGG(+AAAAAA)GTACCAGCCATACTGAGAATCGGGCCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606111945_cy82 GAGCAGGCCTGGCCCGATTCTCAGTATGGCTG(+AAAAAA)GTACCCACCCTACCAGGAAGCACTGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606111944_yxci TCTCTTCAGGGGCCGCTTTCCTGGTGTCAGAG(+AAAAAA)GTACCCATGGTGGAGCAGAGCCACCTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606111942_5bn8 TCTGGGAGGAAGGTGGCTCTGCTCCACCATGG(+AAAAAA)GTACCTCTGACACCAGGAAAGCGGCCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606111940_ffq0 TCTCTAGCACCTGGCTCCTGTGGCTCTCTATG(+AAAAAA)GTACCTGGAATCCCCAAGGTGTTTAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606111938_wjr8 GAGAAAGGAGTTAAACACCTTGGGGATTCCAG(+AAAAAA)GTACCATAGAGAGCCACAGGAGCCAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606111937_y81u ATCTTACAGTCAGCAATCTGGGGGTGGGGAGG(+AAAAAA)GATCCCTCTCTAGCACCTGGCTCCTGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606111935_6bup TAGAGAGCCACAGGAGCCAGGTGCTAGAGAGG(+AAAAAA)GATCCCTCCCCACCCCCAGATTGCTGACTGT NGG 10 8 17 8 24 0 100 5 10
    240606111933_6y75 GGCAAAGACTGAGCTCAGGCACCAGCCTCCCG(+AAAAAA)GTACCACGCTGGAGGTGGATCCCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10