Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111857_if37 TCCGCTCCAATAGAGTAAATAGAGAAGTGCTG(+AAAAAA)GTACCGACGACTCTGGCTGTGCTTGTACACT NGG 10 8 17 8 24 0 100 5 10
    240606111856_w458 TACAGAGTAAGAGCCTGTCTCAAAAAAAAAAG(+AAAAAA)GTACCATTTTTATTGTGAGGGCTCCATGTAG NGG 10 8 17 8 24 0 100 5 10
    240606111854_r635 GAAGGCTACATGGAGCCCTCACAATAAAAATG(+AAAAAA)GTACCTTTTTTTTTTGAGACAGGCTCTTACT NGG 10 8 17 8 24 0 100 5 10
    240606111853_pzme GGGCTAGTGTACAAGCACAGCCAGAGTCGTCG(+AAAAAA)GTACCAGCACTTCTCTATTTACTCTATTGGA NGG 10 8 17 8 24 0 100 5 10
    240606111852_r0hc TCCGCTCCAATAGAGTAAATAGAGAAGTGCTG(+AAAAAA)GTACCGACGACTCTGGCTGTGCTTGTACACT NGG 10 8 17 8 24 0 100 5 10
    240606111851_9bb4 TTCAGGATAGTATGGGAACAAAGAAAGAGAAG(+AAAAAA)GTACCTCTATCCCTGTATCTCCTTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606111850_1e4c GGTGTTCCTTTGAAGGAGATACAGGGATAGAG(+AAAAAA)GTACCTTCTCTTTCTTTGTTCCCATACTATC NGG 10 8 17 8 24 0 100 5 10
    240606111848_9zq5 GCAGGCACAAAACCTTTACTCCAATTCCTTGG(+AAAAAA)GATCCTTCATTCTATCCCTCTCCAGAACTCT NGG 10 8 17 8 24 0 100 5 10
    240606111846_4zm1 TCTCTAGAGTTCTGGAGAGGGATAGAATGAAG(+AAAAAA)GATCCCAAGGAATTGGAGTAAAGGTTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606111845_4ro8 GCAGCAGGCAGGTGGAAGGTAACCACTGTCAG(+AAAAAA)GATCCCCTGAACTGCACGTGTCCTTCCCTAC NGG 10 8 17 8 24 0 100 5 10