Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111931_alo6 TGCCTTCTACTCAGGGATCCACCTCCAGCGTG(+AAAAAA)GTACCGGGAGGCTGGTGCCTGAGCTCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606111930_kjbi GGCACCAGCCTCCCGGTACCACGCTGGAGGTG(+AAAAAA)GATCCCTGAGTAGAAGGCAGGGTAAGCTTCG NGG 10 8 17 8 24 0 100 5 10
    240606111928_2pyp GAAGTCGAAGCTTACCCTGCCTTCTACTCAGG(+AAAAAA)GATCCACCTCCAGCGTGGTACCGGGAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606111927_0th2 CTGTGCCAGAGCCGCTAGCAGCTGCTCACAGG(+AAAAAA)GTACCTTTGTGGGTCTGGTGTTTTGAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240606111925_sbwm GGGTGATTTCTCAAAACACCAGACCCACAAAG(+AAAAAA)GTACCCTGTGAGCAGCTGCTAGCGGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606111923_b2ie GAGACGAGGGGCGGGAGGTCAGCTTCTCCCTG(+AAAAAA)GATCCTCAGTGTGCACTGCCTTGGGCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606111922_sutb GTGGCTCCCTGCCCAAGGCAGTGCACACTGAG(+AAAAAA)GATCCAGGGAGAAGCTGACCTCCCGCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606111920_5ytq GACCCGCGGCTTGCGCATCTGTGACATTTCTG(+AAAAAA)GATCCCGGTCAGTTCCGGCTGTGCCCAGACT NGG 10 8 17 8 24 0 100 5 10
    240606111918_d7gy TCTCCAGTCTGGGCACAGCCGGAACTGACCGG(+AAAAAA)GATCCAGAAATGTCACAGATGCGCAAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240606111916_jszi GTGGTTGCAACTTCTGGAGCAGGTGGGTGTGG(+AAAAAA)GATCCCAGGTGTAGTGTTGGCTGAGCTCAGA NGG 10 8 17 8 24 0 100 5 10