Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112021_qos5 GCGGACGGGCCAGTGTATCTCTCCTCGTGAAG(+AAAAAA)GTACCTCAGGCAACCGCACAGGGATGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606112020_vg9t CAGCAACATCTTCTTCTCAGGGCCACACACTG(+AAAAAA)GATCCTGGGAGAGATGCCCACTTAAGCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606112017_ilg5 ACCCTGGAAGCTTAAGTGGGCATCTCTCCCAG(+AAAAAA)GATCCAGTGTGTGGCCCTGAGAAGAAGATGT NGG 10 8 17 8 24 0 100 5 10
    240606112016_7gp7 CAAGGACAGATGTGACCTGATGATGAAAGAGG(+AAAAAA)GATCCCTCAAGGGCCCAGAAACCTGGGGACA NGG 10 8 17 8 24 0 100 5 10
    240606112014_ipmq TCTCGTGTCCCCAGGTTTCTGGGCCCTTGAGG(+AAAAAA)GATCCCTCTTTCATCATCAGGTCACATCTGT NGG 10 8 17 8 24 0 100 5 10
    240606112012_6gva TGAGAATGCCATGTTTTAGGGACCAGCACCTG(+AAAAAA)GATCCCTGACACTGTAGTCTCATGGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606112010_eg8p GTGCCCAGGGCCCATGAGACTACAGTGTCAGG(+AAAAAA)GATCCAGGTGCTGGTCCCTAAAACATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606112008_6o50 GCTCCCTACACGCAGAGTCACTGGTTCAAGGG(+AAAAAA)GATCCTAGCTGGGATGTATCTCAAGGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606112007_mz7u GGTATTCTACCCTTGAGATACATCCCAGCTAG(+AAAAAA)GATCCCCTTGAACCAGTGACTCTGCGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240606112006_t7ar AACACGGAACTCCCAGGATTCTGGAATGACAG(+AAAAAA)GATCCTCCCAGAAGGTGTGTATACCCCTTAA NGG 10 8 17 8 24 0 100 5 10