Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112004_cyfm TGTGTTTAAGGGGTATACACACCTTCTGGGAG(+AAAAAA)GATCCTGTCATTCCAGAATCCTGGGAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606112003_keaw GGGAAAGTCACATGCTAGCCTCTCCAGCTTCG(+AAAAAA)GATCCAGGGCTCCCTTTGATGTGACAACCAT NGG 10 8 17 8 24 0 100 5 10
    240606112000_7yhi TACCAATGGTTGTCACATCAAAGGGAGCCCTG(+AAAAAA)GATCCGAAGCTGGAGAGGCTAGCATGTGACT NGG 10 8 17 8 24 0 100 5 10
    240606111959_39d6 GTGCCCGGTGTGCAGGCACCAAGGAGACACAG(+AAAAAA)GTACCAGCAGACCACCACTGGTGACATCATC NGG 10 8 17 8 24 0 100 5 10
    240606111958_79z4 CTGGTGATGATGTCACCAGTGGTGGTCTGCTG(+AAAAAA)GTACCTGTGTCTCCTTGGTGCCTGCACACCG NGG 10 8 17 8 24 0 100 5 10
    240606111956_ne6c AGTCCTCACCGAAGTCACATCAACTTCCTATG(+AAAAAA)GTACCTCTCATGAACCTGGGGTGCTTCTTAC NGG 10 8 17 8 24 0 100 5 10
    240606111955_h0u2 ACGGGGTAAGAAGCACCCCAGGTTCATGAGAG(+AAAAAA)GTACCATAGGAAGTTGATGTGACTTCGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606111953_qwcj AGTGCTTGTCTTACATGCACAGGATCTGGTTG(+AAAAAA)GATCCCCATGCATCATTGACACTGGGTATTG NGG 10 8 17 8 24 0 100 5 10
    240606111951_056o ACACCCAATACCCAGTGTCAATGATGCATGGG(+AAAAAA)GATCCAACCAGATCCTGTGCATGTAAGACAA NGG 10 8 17 8 24 0 100 5 10
    240606111950_mno7 CAGCAGGCAGAGGTGAAAAGAACACTGCTGTG(+AAAAAA)GATCCCAATCAAAAGTGTTTTACATGCAAAG NGG 10 8 17 8 24 0 100 5 10