Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111756_755z ACGTGACTGAGCATCAAACTGCACAGGGTCTG(+AAAAAA)GATCCTCACCAACTGGGAAGACCAGCTATGC NGG 10 8 17 8 24 0 100 5 10
    240606111755_03xo TTCACCCTTAGAAAGGGTTGGGGCTACTCTGG(+AAAAAA)GATCCTGAACACCCAGCATAGTCCCTGGATG NGG 10 8 17 8 24 0 100 5 10
    240606111753_nqvz TTAGCCATCCAGGGACTATGCTGGGTGTTCAG(+AAAAAA)GATCCCAGAGTAGCCCCAACCCTTTCTAAGG NGG 10 8 17 8 24 0 100 5 10
    240606111752_5vdq GAAAGCACAAATCCTAAGCACAGCCTTTGAAG(+AAAAAA)GATCCTGCTAATTGTATGTCTTAACAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606111751_osla AAGATTTTATTGTTAAGACATACAATTAGCAG(+AAAAAA)GATCCTTCAAAGGCTGTGCTTAGGATTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606111750_nsc1 ACAAGTACAGTTTCCAGTGCCTCCCTGCTGTG(+AAAAAA)GTACCTGAAAAGTCTGAATGCAAGTAGTATG NGG 10 8 17 8 24 0 100 5 10
    240606111748_f266 ATAGTCATACTACTTGCATTCAGACTTTTCAG(+AAAAAA)GTACCACAGCAGGGAGGCACTGGAAACTGTA NGG 10 8 17 8 24 0 100 5 10
    240606111746_j4gv AAAAATGTTCCTGCAAAGGACATCCACTCCTG(+AAAAAA)GATCCTGATGCCATTCACACAATGAGATAGT NGG 10 8 17 8 24 0 100 5 10
    240606111745_zcxa GCAGCACTATCTCATTGTGTGAATGGCATCAG(+AAAAAA)GATCCAGGAGTGGATGTCCTTTGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240606111743_k8sw TGGGGTGTTTCCTTCCGGCAGAAGCCACCAGG(+AAAAAA)GATCCAAGCTTCATGTGGTTGGCTCCCCAGA NGG 10 8 17 8 24 0 100 5 10