Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111711_y6f0 ACGTCTAAAGAAAAAAATATAAACCTGGCCTG(+AAAAAA)GTACCCATTACATATATACATAATATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240606111709_7c7y GCTCACAACCATGCATAACTCAAGTTTTAAGG(+AAAAAA)GATCCAGTGCTGACTTCTGGCCTCTGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240606111708_wm1r GCAGTACCCTCAGAGGCCAGAAGTCAGCACTG(+AAAAAA)GATCCCTTAAAACTTGAGTTATGCATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606111706_bt2n TTGGGGGAGATGCAGCACACACCCCAGATAGG(+AAAAAA)GATCCTATGACTGACCAACAAAAGTACAGGT NGG 10 8 17 8 24 0 100 5 10
    240606111705_n9x2 GTGGTACCTGTACTTTTGTTGGTCAGTCATAG(+AAAAAA)GATCCCTATCTGGGGTGTGTGCTGCATCTCC NGG 10 8 17 8 24 0 100 5 10
    240606111703_cd5x CATGCCATTTTAGGGCCATTGTGATTCTCAGG(+AAAAAA)GATCCTAGGTAAAAATCCTGAATCTTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606111701_t5gi TCTTACCCAAAAGATTCAGGATTTTTACCTAG(+AAAAAA)GATCCCTGAGAATCACAATGGCCCTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606111700_7neb TCTTGCTTGTCAACTTCAGAGATAGCTGGAAG(+AAAAAA)GATCCTGAGACTGTATGGCAGGAGAGTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606111659_5zzb AACCCCTCAACTCTCCTGCCATACAGTCTCAG(+AAAAAA)GATCCTTCCAGCTATCTCTGAAGTTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240606111657_fxww AAAAGCATGAGATTTGCATCCCAGCTGTCCTG(+AAAAAA)GATCCAGGCTGTGAGGTTTTGAGGATTCTTA NGG 10 8 17 8 24 0 100 5 10