Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111628_zs76 ACTTCCTTTTCCCTACATCTTTCAGTATGAGG(+AAAAAA)GATCCATATCCTGCTCCACCCAGGTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606111627_8zx4 GGGGTACGTTATCTACTGCCTTCTCCCTTCTG(+AAAAAA)GATCCACGATAAAGCTCTGGCTTTAATAAAC NGG 10 8 17 8 24 0 100 5 10
    240606111626_qs55 AACCAGTTTATTAAAGCCAGAGCTTTATCGTG(+AAAAAA)GATCCAGAAGGGAGAAGGCAGTAGATAACGT NGG 10 8 17 8 24 0 100 5 10
    240606111624_fc7p CCCTGCGGTGGGAGGGGCAGGCAGGGGAGGTG(+AAAAAA)GTACCCTCCCACCAACCTGAGCCCACTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606111623_fnxh TCTGAACAAAAGGAAGGAAAATGATTGGACAG(+AAAAAA)GTACCCCACAAACTTTCCATCTAAGTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240606111621_lzb9 GTTGGGCAACACTTAGATGGAAAGTTTGTGGG(+AAAAAA)GTACCTGTCCAATCATTTTCCTTCCTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606111620_yild CCCTAGCCTAGGGCTTCGACATTGCATGCTGG(+AAAAAA)GATCCAGCTCCTACCCTTCCTGCACTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240606111619_y9se TGGGGAAGGGAGTGCAGGAAGGGTAGGAGCTG(+AAAAAA)GATCCCAGCATGCAATGTCGAAGCCCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240606111618_6on8 CTAACCACTGCCCTCCTGGAACTGCTGTGCTG(+AAAAAA)GATCCAAAGGCCTCCTCACCAGGAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606111616_5hkf GGGCCAGAGCCTTCCTGGTGAGGAGGCCTTTG(+AAAAAA)GATCCAGCACAGCAGTTCCAGGAGGGCAGTG NGG 10 8 17 8 24 0 100 5 10