Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111540_7sx9 CTTTGCCAGCCCCCTACCCCTTTATTATCTCG(+AAAAAA)GATCCCATCTTCTCCAGCAGCAGGCACCAAG NGG 10 8 17 8 24 0 100 5 10
    240606111538_rbp9 CTCGCCTTGGTGCCTGCTGCTGGAGAAGATGG(+AAAAAA)GATCCGAGATAATAAAGGGGTAGGGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606111537_tg5d ACTGTTGTTGTCTTCAGACACACCAGAAGAGG(+AAAAAA)GATCCCATTATGGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606111535_56gk ACCACATGGTGGCTCACAACCATCCATAATGG(+AAAAAA)GATCCCTCTTCTGGTGTGTCTGAAGACAACA NGG 10 8 17 8 24 0 100 5 10
    240606111534_2yll TTTCTATGTAATCCAAGTGCCTCCTCCGGCTG(+AAAAAA)GTACCAGGATGGGAACAGAAGCCGGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240606111532_urnc CACCTGATAAACCGGCTTCTGTTCCCATCCTG(+AAAAAA)GTACCAGCCGGAGGAGGCACTTGGATTACAT NGG 10 8 17 8 24 0 100 5 10
    240606111531_fl30 CTAGGCTAGGAGCTGGAGCTGTCTCCCTCCTG(+AAAAAA)GATCCAATAAAGCCAGATGCTTTGCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606111529_vstt TGGAGCTGGAGGCAAAGCATCTGGCTTTATTG(+AAAAAA)GATCCAGGAGGGAGACAGCTCCAGCTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240606111527_oou3 CTATCTGCACACTGACAACAAGAGGGCCCTGG(+AAAAAA)GATCCAGTGGGAAATGACGTGGGAATGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606111525_j3ne AAAAACCTTCATTCCCACGTCATTTCCCACTG(+AAAAAA)GATCCCAGGGCCCTCTTGTTGTCAGTGTGCA NGG 10 8 17 8 24 0 100 5 10