Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111435_kdxf CAGATGCCTCCAGCACTGCCATGTGCCCAGTG(+AAAAAA)GTACCCCTTGACAGTGTTGTTCTCAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240606111433_c648 GAACAAGTCACTGAGAACAACACTGTCAAGGG(+AAAAAA)GTACCACTGGGCACATGGCAGTGCTGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606111432_ot0y ATAGACTCGGATCCTTCCTGCATTGTTGCAAG(+AAAAAA)GTACCCAATTGTCAGTTGCAGGAGACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606111431_z3l9 CACACTCTGGGTCTCCTGCAACTGACAATTGG(+AAAAAA)GTACCTTGCAACAATGCAGGAAGGATCCGAG NGG 10 8 17 8 24 0 100 5 10
    240606111430_cn52 TCTGCATAGTTTAAAACCACATGGATCATCTG(+AAAAAA)GTACCTTTTTTTCCTAGACAACTTTGATCCT NGG 10 8 17 8 24 0 100 5 10
    240606111428_y6ny AGACAAGGATCAAAGTTGTCTAGGAAAAAAAG(+AAAAAA)GTACCAGATGATCCATGTGGTTTTAAACTAT NGG 10 8 17 8 24 0 100 5 10
    240606111426_9fvy AACCCCCCACAGATGGTGTGTTGAAACCTTGG(+AAAAAA)GATCCCAGCATACAGAGTATACTTTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606111425_g88l AAATTCAACAAAAAGTATACTCTGTATGCTGG(+AAAAAA)GATCCCAAGGTTTCAACACACCATCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606111423_sjzm GAACTTGGCCTTTGAAACTCATCATAGACTCG(+AAAAAA)GATCCTTCCTGCATTGTTGCAAGGTACCCAA NGG 10 8 17 8 24 0 100 5 10
    240606111422_e0z1 GACAATTGGGTACCTTGCAACAATGCAGGAAG(+AAAAAA)GATCCGAGTCTATGATGAGTTTCAAAGGCCA NGG 10 8 17 8 24 0 100 5 10