Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111524_vw2q ATCACAATCATCTGTAACTTAGGTTCCAGAGG(+AAAAAA)GATCCAATGCCCTCTTCTGACTTCTGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606111522_z5p1 TTACCGGCTGCAGAAGTCAGAAGAGGGCATTG(+AAAAAA)GATCCCTCTGGAACCTAAGTTACAGATGATT NGG 10 8 17 8 24 0 100 5 10
    240606111521_hedh AATGTCTGAACCCTGGCTTTCAATTTTTTTAG(+AAAAAA)GTACCCTACTTAGAGATGGACTTGCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240606111520_nh6e CATGTAATCCAGCAAGTCCATCTCTAAGTAGG(+AAAAAA)GTACCTAAAAAAATTGAAAGCCAGGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606111518_tx9f ATCTGACTGAGATTTGCCAAGTGGATACTGGG(+AAAAAA)GTACCACTGCCCCCCAAGAAAAGACTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606111512_ygq1 TTCCTGGCTCAGTCTTTTCTTGGGGGGCAGTG(+AAAAAA)GTACCCCAGTATCCACTTGGCAAATCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606111511_ddwf CACAGGGCCCAGATGGTGGGTAACTGAGGCAG(+AAAAAA)GATCCAGACTTGAGACACACGGTTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240606111510_atyf AGCCTTGTTTCCAACCGTGTGTCTCAAGTCTG(+AAAAAA)GATCCTGCCTCAGTTACCCACCATCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606111508_4yzl GCCTTGTCTAGGAACACAGAAAGGAGATGATG(+AAAAAA)GTACCTACTGGCCTTGGACTCTTCTGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240606111507_217p GTTTGTCACGCAGAAGAGTCCAAGGCCAGTAG(+AAAAAA)GTACCATCATCTCCTTTCTGTGTTCCTAGAC NGG 10 8 17 8 24 0 100 5 10