Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111556_yemo GCTCAGCAGGAAGGCATATTTCAAAAACCAGG(+AAAAAA)GTACCCTAGAGAACTTTTCAGATCACAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606111554_js8u TCTGCGGCTTGTGATCTGAAAAGTTCTCTAGG(+AAAAAA)GTACCCTGGTTTTTGAAATATGCCTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240606111553_2cra AGGACCTCCAGTCAGCCCACACACCTCAGCGG(+AAAAAA)GTACCTTGCCCCTTTTCTCCAGTACAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606111551_8aok AGTGCTGAACTGTACTGGAGAAAAGGGGCAAG(+AAAAAA)GTACCCGCTGAGGTGTGTGGGCTGACTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606111550_0v48 CTAGAGAACGGGGTCAAGTAGTCTACCATTTG(+AAAAAA)GATCCAGGCGCTTACTCCGTTTGAGAAGACA NGG 10 8 17 8 24 0 100 5 10
    240606111549_b7u5 CCAGGTGTCTTCTCAAACGGAGTAAGCGCCTG(+AAAAAA)GATCCAAATGGTAGACTACTTGACCCCGTTC NGG 10 8 17 8 24 0 100 5 10
    240606111546_vp1p GTCAGGAGCCCAATTCCTGGGAGCAGTAGGGG(+AAAAAA)GATCCAGGACCTCCAGTCAGCCCACACACCT NGG 10 8 17 8 24 0 100 5 10
    240606111545_itew CGCTGAGGTGTGTGGGCTGACTGGAGGTCCTG(+AAAAAA)GATCCCCCTACTGCTCCCAGGAATTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606111543_49vg TGCCCAGGGGCGCGCGCTCTTAATTGACTCGG(+AAAAAA)GATCCTTGGAACGATCCCCTTCTTGCCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606111541_kbih TGTCATAAAGGCAAGAAGGGGATCGTTCCAAG(+AAAAAA)GATCCCGAGTCAATTAAGAGCGCGCGCCCCT NGG 10 8 17 8 24 0 100 5 10