Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111642_k1cs AGAGCAGATTAGGACACCACCTGACAGAACAG(+AAAAAA)GTACCTTGATGAGGTTTATCAGTAACTCGGT NGG 10 8 17 8 24 0 100 5 10
    240606111641_f09o TGTCCACACATCCCTCTCTAAGCCCAGGATAG(+AAAAAA)GTACCAATATAGGTAACTCTGCATTTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606111640_im40 CTTATGGGAAAAATGCAGAGTTACCTATATTG(+AAAAAA)GTACCTATCCTGGGCTTAGAGAGGGATGTGT NGG 10 8 17 8 24 0 100 5 10
    240606111639_x4zg TACACCTCTAGGAACTGAGGCAGCTTCAGAAG(+AAAAAA)GTACCTGCCAGATGTTGCTGTGGGAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606111638_40pn AAGGCCATGCTTCCCACAGCAACATCTGGCAG(+AAAAAA)GTACCTTCTGAAGCTGCCTCAGTTCCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240606111635_dari GATGGCTTACAACTGTAACCCTGGTTCCAGGG(+AAAAAA)GATCCAGTACCTTCTGACCTGCTCGGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240606111634_i50j TTCCTGGCACCCGAGCAGGTCAGAAGGTACTG(+AAAAAA)GATCCCCTGGAACCAGGGTTACAGTTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240606111632_q2dl TTCCTTGAAGTGAGGTCAAGGTCATGTGGCCG(+AAAAAA)GATCCTATACTCACCACTGGCTGTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606111631_mky8 CTTGAGAGAAAGACAGCCAGTGGTGAGTATAG(+AAAAAA)GATCCGGCCACATGACCTTGACCTCACTTCA NGG 10 8 17 8 24 0 100 5 10
    240606111630_3p1z TTATCCACAGGACCTGGGTGGAGCAGGATATG(+AAAAAA)GATCCCTCATACTGAAAGATGTAGGGAAAAG NGG 10 8 17 8 24 0 100 5 10