Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113937_ccyt ATTGCACTTATTTCTATGAACCCCATGGAGGG(+AAAAAA)GATCCCCAATGCTGCTGCCTCCAGGCGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606113935_p0b4 GTCATGCCTCGCCTGGAGGCAGCAGCATTGGG(+AAAAAA)GATCCCCTCCATGGGGTTCATAGAAATAAGT NGG 10 8 17 8 24 0 100 5 10
    240606113934_gdzv GGTACTTCCTGCAGAGCCTGACACCAGAGTTG(+AAAAAA)GATCCCTGGGACCCACATGATGAGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240606113931_wfh6 GAAGCTGGATCTCTCATCATGTGGGTCCCAGG(+AAAAAA)GATCCAACTCTGGTGTCAGGCTCTGCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606113929_uagp CAGATTGATGTTCAACATGGGGTCTGAGTAGG(+AAAAAA)GATCCCAACTAAAAAGCCATAACCTTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606113928_lzpx TTCAGTTCTGAAGGTTATGGCTTTTTAGTTGG(+AAAAAA)GATCCCTACTCAGACCCCATGTTGAACATCA NGG 10 8 17 8 24 0 100 5 10
    240606113927_erl4 TGTTGCTGTGAGGTCTTATGGCAGCTGCTCTG(+AAAAAA)GATCCATTGCTGGCTTCTTTGGCACCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606113925_4kuu TGAACCTGGAGGTGCCAAAGAAGCCAGCAATG(+AAAAAA)GATCCAGAGCAGCTGCCATAAGACCTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606113924_7qni GGTTTGTCCAAATGTGTGGCATGGGAGATAGG(+AAAAAA)GTACCCCTGAAAGCAAGGAAAGGAGCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606113922_csa7 CCACAGCTGGGCTCCTTTCCTTGCTTTCAGGG(+AAAAAA)GTACCCTATCTCCCATGCCACACATTTGGAC NGG 10 8 17 8 24 0 100 5 10