Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112220_wabw GGCCACTACCACTAAGGCTTTGAGTTTGTGTG(+AAAAAA)GATCCTTGAGAGCTAAGTTCAGGTGACCTTG NGG 10 8 17 8 24 0 100 5 10
    240606112219_jm5h ACACAGCAATGGGAATAGCCACCGAGTTTAAG(+AAAAAA)GTACCAGGTGGCAACAAACCAATAATAACTT NGG 10 8 17 8 24 0 100 5 10
    240606112218_p84x TAGTTAAGTTATTATTGGTTTGTTGCCACCTG(+AAAAAA)GTACCTTAAACTCGGTGGCTATTCCCATTGC NGG 10 8 17 8 24 0 100 5 10
    240606112217_acxc GTGGGCATATGTGGGTGCCAGGCTGGAACAAG(+AAAAAA)GTACCACTTTTAGAGTGAGATGTGACATTGT NGG 10 8 17 8 24 0 100 5 10
    240606112215_gjmi ACTCAACAATGTCACATCTCACTCTAAAAGTG(+AAAAAA)GTACCTTGTTCCAGCCTGGCACCCACATATG NGG 10 8 17 8 24 0 100 5 10
    240606112214_yes9 CAACTTAGGGGGTGAGCAGGTGTTGGTGATGG(+AAAAAA)GATCCCTGTGATGAGATGGGGGTGACACTGC NGG 10 8 17 8 24 0 100 5 10
    240606112212_n6h4 AATGTGCAGTGTCACCCCCATCTCATCACAGG(+AAAAAA)GATCCCATCACCAACACCTGCTCACCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240606112210_0eka TCGAATATTTAGAAGTGTAAAACATAAACCAG(+AAAAAA)GATCCTGCCCCCCACATGTCCAGGACGATGA NGG 10 8 17 8 24 0 100 5 10
    240606112209_k4tt AACACTCATCGTCCTGGACATGTGGGGGGCAG(+AAAAAA)GATCCTGGTTTATGTTTTACACTTCTAAATA NGG 10 8 17 8 24 0 100 5 10
    240606112208_f5vc TAAATTTCACCCTCTGCATTACTCACAGAGTG(+AAAAAA)GATCCATTGGAGTTTCAGCTGTATGCTAGTT NGG 10 8 17 8 24 0 100 5 10