Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606112233_amu0 ATTTAAAATGTTTTCTGACCTTGATAAAAATG(+AAAAAA)GATCCATTAAATATCTTATTAAATAAAAACT NGG 10 8 17 8 24 0 100 5 10
    240606112232_qvk5 AGGACAAAGGCTGGAAACTTCAGATGAACTTG(+AAAAAA)GTACCCACTGAGACTTCCCTGCTGAAAGACA NGG 10 8 17 8 24 0 100 5 10
    240606112231_yr0m ACCACTGTCTTTCAGCAGGGAAGTCTCAGTGG(+AAAAAA)GTACCAAGTTCATCTGAAGTTTCCAGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240606112229_i6m3 AAGAAGCCCGGCGGAGCTTTAGGTGGCCACAG(+AAAAAA)GTACCTGCTGGCAGTGCTGTCACATTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240606112228_s4g1 CCTTGCCTTAAATGTGACAGCACTGCCAGCAG(+AAAAAA)GTACCTGTGGCCACCTAAAGCTCCGCCGGGC NGG 10 8 17 8 24 0 100 5 10
    240606112227_jy4k ACTTGGCTGAGGAGACTCGGCTTAGGAAGCAG(+AAAAAA)GTACCTGACTTTTGAGAAAACCCACTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606112226_22d0 AACAGAAGCTAGTGGGTTTTCTCAAAAGTCAG(+AAAAAA)GTACCTGCTTCCTAAGCCGAGTCTCCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606112224_igth AGGAATCGATTCATAGTCCTGGGTATCAAAGG(+AAAAAA)GATCCAGTCACCAGGAGCTCCTCTCTTACCA NGG 10 8 17 8 24 0 100 5 10
    240606112223_aesx ATCCCTGGTAAGAGAGGAGCTCCTGGTGACTG(+AAAAAA)GATCCCTTTGATACCCAGGACTATGAATCGA NGG 10 8 17 8 24 0 100 5 10
    240606112222_il7v GGGGACAAGGTCACCTGAACTTAGCTCTCAAG(+AAAAAA)GATCCACACAAACTCAAAGCCTTAGTGGTAG NGG 10 8 17 8 24 0 100 5 10