Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606110949_znb9 CTCTCCTTCTCCAGTGCTTATAGACATGTATG(+AAAAAA)GATCCTGTTCTGCTGCTGGGAAAGCCACTGG NGG 10 8 17 8 24 0 100 5 10
    240606110948_j46o GTCCCCCAGTGGCTTTCCCAGCAGCAGAACAG(+AAAAAA)GATCCATACATGTCTATAAGCACTGGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606110946_37eg CCCATCAGTCTTTAGCCCCAGGCTCAGGCAGG(+AAAAAA)GATCCCTTCATCTGATCCTCTACCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606110945_0wjh GTGGGGAGGAGGGGTAGAGGATCAGATGAAGG(+AAAAAA)GATCCCTGCCTGAGCCTGGGGCTAAAGACTG NGG 10 8 17 8 24 0 100 5 10
    240606110943_49va GCTCTCGCAGAAGATGGAGGTTCAATCTCCAG(+AAAAAA)GATCCAGATGATGGCTCCCAACTGTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240606110942_ocwf TGGAGTTACAGACAGTTGGGAGCCATCATCTG(+AAAAAA)GATCCTGGAGATTGAACCTCCATCTTCTGCG NGG 10 8 17 8 24 0 100 5 10
    240606110940_ihm3 ACACACACACACCACACGCAAAGCCATGGGAG(+AAAAAA)GATCCCAGAGGCCTTGTTGAGAATTGGATTC NGG 10 8 17 8 24 0 100 5 10
    240606110939_po0g CTCATGAATCCAATTCTCAACAAGGCCTCTGG(+AAAAAA)GATCCTCCCATGGCTTTGCGTGTGGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606110937_lble ACAAGGACACTGGGAGGTGTGTGAACGCCATG(+AAAAAA)GTACCGGCGGCTGCTTGAGGCGTTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606110934_f0h1 GAGTCACAGGAAAACGCCTCAAGCAGCCGCCG(+AAAAAA)GTACCATGGCGTTCACACACCTCCCAGTGTC NGG 10 8 17 8 24 0 100 5 10