Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606110919_apfj GCTGCCTGCAGAGCCCAGACATCTCTAGGAGG(+AAAAAA)GATCCACTCAACGGCCTAATCTTACCTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606110918_4t6u AGGTAATCTAGGTAAGATTAGGCCGTTGAGTG(+AAAAAA)GATCCCTCCTAGAGATGTCTGGGCTCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606110917_6tck TTCTTGGTGGTAAAGGCGCCATGCTGTCTTTG(+AAAAAA)GTACCTGACAGGTTTTAGCAGCCTAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606110915_uxp3 AAAGGTCTTGCTAGGCTGCTAAAACCTGTCAG(+AAAAAA)GTACCAAAGACAGCATGGCGCCTTTACCACC NGG 10 8 17 8 24 0 100 5 10
    240606110913_aga1 GTACAAGGGCTTTGCTGATAGTTAGCTGGTGG(+AAAAAA)GTACCACTGACGCTGAGAGCATGCCACCAGA NGG 10 8 17 8 24 0 100 5 10
    240606110912_a8y4 AGGGCTCTGGTGGCATGCTCTCAGCGTCAGTG(+AAAAAA)GTACCCACCAGCTAACTATCAGCAAAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606110910_ubrs TTCCACAGTGGCATGGCCACCCAGCCTCGGGG(+AAAAAA)GATCCTGGGCTCGCTGCACAGCACCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240606110908_w91d GAAGAGATCTGGGTGCTGTGCAGCGAGCCCAG(+AAAAAA)GATCCCCCGAGGCTGGGTGGCCATGCCACTG NGG 10 8 17 8 24 0 100 5 10
    240606110907_w6w5 AGCCTCCACACGAGTGTTCTAGTACATGTTAG(+AAAAAA)GATCCTCTCCTGTACCAGTGTCCAGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240606110906_6etm GTCTGTTACACCTGGACACTGGTACAGGAGAG(+AAAAAA)GATCCTAACATGTACTAGAACACTCGTGTGG NGG 10 8 17 8 24 0 100 5 10