Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606110815_k8rm GGCTGATGCCTGTCAAGATGACAACAGGAGGG(+AAAAAA)GATCCACCAAGGTAGAAAACATCCCTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606110814_n5lt TCTTGTCAGCAGGGATGTTTTCTACCTTGGTG(+AAAAAA)GATCCCCTCCTGTTGTCATCTTGACAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606110812_k85g AGGTGAACGTTCCACTGTAAAAGGCACTACTG(+AAAAAA)GATCCGAGAGGGTTTATTTTAAGCATGTGCA NGG 10 8 17 8 24 0 100 5 10
    240606110811_6izp TCTGCTGCACATGCTTAAAATAAACCCTCTCG(+AAAAAA)GATCCAGTAGTGCCTTTTACAGTGGAACGTT NGG 10 8 17 8 24 0 100 5 10
    240606110809_dpll GAACATGGGGGGTGAAATGGTGCTGAGGGGAG(+AAAAAA)GTACCGACTCAGTGTCCACGCTCACTGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606110808_u7c9 AGGACATGACAGTGAGCGTGGACACTGAGTCG(+AAAAAA)GTACCTCCCCTCAGCACCATTTCACCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606110807_rwue AATATAAATATGTGACAGAGACATTATGGTGG(+AAAAAA)GTACCACCTTAAAAAAACATCTGGTAGCATT NGG 10 8 17 8 24 0 100 5 10
    240606110805_6qnd AATAAAATGCTACCAGATGTTTTTTTAAGGTG(+AAAAAA)GTACCCACCATAATGTCTCTGTCACATATTT NGG 10 8 17 8 24 0 100 5 10
    240606110804_1cqv TGAAATTTTCATTTTGTTTAAAAAACAATACG(+AAAAAA)GTACCTTGTTCCCTAAAACAGTCTGCACTTA NGG 10 8 17 8 24 0 100 5 10
    240606110802_rcgw ACTTCTAAGTGCAGACTGTTTTAGGGAACAAG(+AAAAAA)GTACCGTATTGTTTTTTAAACAAAATGAAAA NGG 10 8 17 8 24 0 100 5 10