Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605232101_kkbr ATTGAAAAAAAATGGTTGTTTTCCTGTGTTGG(+CAGTGT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605232059_6wqu ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+CAGTGT)GTACCCAACACAGGAAAACAACCATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605232057_eiyh GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+CAGTGT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605232056_va9q ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+CAGTGT)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605232054_n7x3 ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+CAGTGT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605232052_7j0a ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+CAGTGT)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605232050_1x3u ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+CAGTGT)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605232049_2g0z ATTGAAAAAAAATGTTTATTTTCCTGTGTTGG(+CAGTGT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605232046_ltde ATTGAAAAAAAATGGTTGTTTTCCTGTGTTGG(+CAGTGT)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605232044_9mf9 ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+CAGTGT)GTACCCAACACAGGAAAACAACCATTTTTTT NGG 10 8 17 8 24 0 100 5 10