Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606110742_ovn7 TAAGCCTTCCTTCATTCTGAATACTTCACTTG(+AAAAAA)GTACCATGCTAGGACTGAGAGTCATATTTCA NGG 10 8 17 8 24 0 100 5 10
    240606110741_yqf0 CTTTGTGAAATATGACTCTCAGTCCTAGCATG(+AAAAAA)GTACCAAGTGAAGTATTCAGAATGAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606110739_5pcr ACTGCGATTCTCCAATGGGTTACGTGCCAATG(+AAAAAA)GTACCTACAATGTATTGTTATAAGACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606110738_kh6g TCCATTCTAGGTCTTATAACAATACATTGTAG(+AAAAAA)GTACCATTGGCACGTAACCCATTGGAGAATC NGG 10 8 17 8 24 0 100 5 10
    240606110736_lg0g CTGTGGTTAGTCACAGATGCAAGAGATCCAAG(+AAAAAA)GATCCGAGGCTTTGTGAGCACAGTAATTAAG NGG 10 8 17 8 24 0 100 5 10
    240606110734_104g TTACTCTTAATTACTGTGCTCACAAAGCCTCG(+AAAAAA)GATCCTTGGATCTCTTGCATCTGTGACTAAC NGG 10 8 17 8 24 0 100 5 10
    240606110733_i5t9 CTTAGAATTCTCAATGGAAGAGAATGGTACAG(+AAAAAA)GTACCAACAACATGCCATATGCATAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606110732_r5k5 CTGTGCTGCTCTATGCATATGGCATGTTGTTG(+AAAAAA)GTACCTGTACCATTCTCTTCCATTGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606110730_tlod ATGTAATAGTGTCCAGCCAAACAATCCAACAG(+AAAAAA)GATCCTCCACTCTTCTTTCATGTAAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606110729_bbi3 TAACTTACAACTTACATGAAAGAAGAGTGGAG(+AAAAAA)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10