Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606110844_7oux TCTTTCTCTAGCTCTTCCATTGGGGGACCTGG(+AAAAAA)GATCCATCCAATAGCTGACTGTGAGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240606110842_jxpj AGAAGTGGATGCTCACAGTCAGCTATTGGATG(+AAAAAA)GATCCCAGGTCCCCCAATGGAAGAGCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240606110841_par2 CTAATGGTGCGTTTGTTTCTGCTTTTGACTTG(+AAAAAA)GTACCTGTGCACTCTAAGCAGGTAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606110840_4jl2 TTCCCCAGGGCCTACCTGCTTAGAGTGCACAG(+AAAAAA)GTACCAAGTCAAAAGCAGAAACAAACGCACC NGG 10 8 17 8 24 0 100 5 10
    240606110838_g4dz GACAGCTCCTTCAGGCAGTAGCTGTGAAGGAG(+AAAAAA)GTACCTGTGTCTCAGAAATGGCGTGTCCACA NGG 10 8 17 8 24 0 100 5 10
    240606110837_t28l AAACATGTGGACACGCCATTTCTGAGACACAG(+AAAAAA)GTACCTCCTTCACAGCTACTGCCTGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606110836_zl2d ACCAAGGGGGGAAATCCCAGCAGATAATTTAG(+AAAAAA)GATCCCCTTCTAAACACATGAGGGCCTGTAT NGG 10 8 17 8 24 0 100 5 10
    240606110834_5t0u TGCACATACAGGCCCTCATGTGTTTAGAAGGG(+AAAAAA)GATCCTAAATTATCTGCTGGGATTTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606110833_kh0q AATGTTTAGAGAACAATTAGGTCAATTTCAGG(+AAAAAA)GATCCACTTGGTGTATGTGTGGACCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240606110831_hvko ACCTATCTGTAGGTCCACACATACACCAAGTG(+AAAAAA)GATCCCTGAAATTGACCTAATTGTTCTCTAA NGG 10 8 17 8 24 0 100 5 10