Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225817_jvcu CAGCACCTTAATGATCGCCGTTGGCCACGTGG(+CAGTGT)GATCCCCAATGCAAACATATTTATAGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605225815_4r11 CGCACTCCACCTATAAATATGTTTGCATTGGG(+CAGTGT)GATCCCACGTGGCCAACGGCGATCATTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605225812_yrdx GTACAGCAGTCAGACTAGAACTGAACGTCGGG(+CAGTGT)GATCCAGGGCGCGGGCAGTGGAAACGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605225809_y1dq TCCCTGCCTGCGTTTCCACTGCCCGCGCCCTG(+CAGTGT)GATCCCCGACGTTCAGTTCTAGTCTGACTGC NGG 10 8 17 8 24 0 100 5 10
    240605225808_pbcw TGCCTGACGGAGCTTTGGGCGCAGCTGGAAAG(+CAGTGT)GATCCGTAGAGCAGTGGCTTCCCTTGTATGC NGG 10 8 17 8 24 0 100 5 10
    240605225807_qf7p TGCACGCATACAAGGGAAGCCACTGCTCTACG(+CAGTGT)GATCCTTTCCAGCTGCGCCCAAAGCTCCGTC NGG 10 8 17 8 24 0 100 5 10
    240605225806_uqgx GGTGCCTCTCTCCCCCCTTAGACATCAGACAG(+CAGTGT)GATCCCTTTATAGAATAGCCAAGTAAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605225804_d46c CACTGGTTTCTTACTTGGCTATTCTATAAAGG(+CAGTGT)GATCCTGTCTGATGTCTAAGGGGGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605225802_c7a5 CAGCACTGCTCCCTGGGTGCTGAGCACTGGTG(+CAGTGT)GATCCAGTGCCCCAGAGGGCAAGTCCCTTCG NGG 10 8 17 8 24 0 100 5 10
    240605225801_orka AGCTGCGAAGGGACTTGCCCTCTGGGGCACTG(+CAGTGT)GATCCACCAGTGCTCAGCACCCAGGGAGCAG NGG 10 8 17 8 24 0 100 5 10