Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225725_s9iy AGTCAGGGTAGGACCAGCTCCGGTTGTTGGGG(+CAGTGT)GATCCGATGAAGACCAGCCTGTACATCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605225724_pddc TATGTAGCAGATGTACAGGCTGGTCTTCATCG(+CAGTGT)GATCCCCCAACAACCGGAGCTGGTCCTACCC NGG 10 8 17 8 24 0 100 5 10
    240605225723_hj63 GAGCTTGCGCTTGCTTGCTAAACCTCTAACAG(+CAGTGT)GTACCTGGGGATGATGGTGCACACCTATAAC NGG 10 8 17 8 24 0 100 5 10
    240605225720_t6t6 TTAGGGTTATAGGTGTGCACCATCATCCCCAG(+CAGTGT)GTACCTGTTAGAGGTTTAGCAAGCAAGCGCA NGG 10 8 17 8 24 0 100 5 10
    240605225719_lm4u GGACAAAAAAAAAAGAAAAGAAAAGAAAAAAG(+CAGTGT)GATCCCCCCCTTCTGTTTTTGTTCCTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605225716_xt9p AGAAATCACGAGGAACAAAAACAGAAGGGGGG(+CAGTGT)GATCCTTTTTTCTTTTCTTTTCTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605225715_kgkx AGTTGATAAGTTTTCTGATATTCTGATATTAG(+CAGTGT)GTACCACGGATGCAGGAATATTACTTGATAT NGG 10 8 17 8 24 0 100 5 10
    240605225712_6rtr AGCCAATATCAAGTAATATTCCTGCATCCGTG(+CAGTGT)GTACCTAATATCAGAATATCAGAAAACTTAT NGG 10 8 17 8 24 0 100 5 10
    240605225710_1efj GCACCTGCTTGGCTGTGACTCAGGTCCCAGGG(+CAGTGT)GATCCAGATCCCTTCTCTGACCTTTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605225709_f8jq CTGGTGCCAGCAAAGGTCAGAGAAGGGATCTG(+CAGTGT)GATCCCCTGGGACCTGAGTCACAGCCAAGCA NGG 10 8 17 8 24 0 100 5 10