Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225635_n2j9 ATTAGGAGCCTTAGGCCCTCACTGGGGTCCAG(+CAGTGT)GATCCCAGGGGTAAGTCTAAAGTGCCATTGG NGG 10 8 17 8 24 0 100 5 10
    240605225632_052w AGACCCCAATGGCACTTTAGACTTACCCCTGG(+CAGTGT)GATCCTGGACCCCAGTGAGGGCCTAAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605225630_tz6k CCAACTTCTCTGGAACATATTGTTTCACACTG(+CAGTGT)GATCCTTCAACAAAGGATTGAATGGCTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605225629_fv3z CTTTCATAGAGCCATTCAATCCTTTGTTGAAG(+CAGTGT)GATCCAGTGTGAAACAATATGTTCCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605225628_9tfl CCCACTCCTAACCAAGAAGCAGTGTGCAGTTG(+CAGTGT)GTACCTGCTAGAAAGGGGAAATCACTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605225626_rpfw GATGGAACTCAGTGATTTCCCCTTTCTAGCAG(+CAGTGT)GTACCAACTGCACACTGCTTCTTGGTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240605225625_q09w ACATCAAAAGCTCTCAAACAGCAGCCACACTG(+CAGTGT)GATCCCAGCACTGAGAGGGCTAAGTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605225622_65fe GGCCTGTGCCCACTTAGCCCTCTCAGTGCTGG(+CAGTGT)GATCCAGTGTGGCTGCTGTTTGAGAGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605225621_o58d CGTGGCAGCTGCACAGCTCTGCCTTTAGTTTG(+CAGTGT)GATCCTGTTTGCTTTGAGCACAGGTTTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605225620_s1kr ACCAGTCACAAACCTGTGCTCAAAGCAAACAG(+CAGTGT)GATCCAAACTAAAGGCAGAGCTGTGCAGCTG NGG 10 8 17 8 24 0 100 5 10