Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225651_a2ks AACTTAAATAAAAATAGCACCAGAAGGACCAG(+CAGTGT)GTACCAATAACATATTTCACCCATCCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240605225649_kf9i TAAAATTACTGGATGGGTGAAATATGTTATTG(+CAGTGT)GTACCTGGTCCTTCTGGTGCTATTTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605225648_vvrh TACAAACTAATTTTTAGTAGCTGATAATACAG(+CAGTGT)GATCCCACAGTCTATTTACATAAAAGCCCGT NGG 10 8 17 8 24 0 100 5 10
    240605225647_e7vk TAAAAACGGGCTTTTATGTAAATAGACTGTGG(+CAGTGT)GATCCTGTATTATCAGCTACTAAAAATTAGT NGG 10 8 17 8 24 0 100 5 10
    240605225645_2mlm TCTCTGGAGAGACCCTGGCCCCAGGACAACAG(+CAGTGT)GTACCAGGTTTCCCAGCTATCTCATTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605225644_zxct CTGGCAGAGAAATGAGATAGCTGGGAAACCTG(+CAGTGT)GTACCTGTTGTCCTGGGGCCAGGGTCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605225641_vn9s GTATTCCAGGGTGTCAGGATTAGGAGGCCTGG(+CAGTGT)GTACCCCTGCTCTAGACTCCCCTTCCTTATG NGG 10 8 17 8 24 0 100 5 10
    240605225639_l634 GGAATCATAAGGAAGGGGAGTCTAGAGCAGGG(+CAGTGT)GTACCCAGGCCTCCTAATCCTGACACCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605225638_6eii TTCCATCATTGTATCTCCTTTCCGCTGTGCGG(+CAGTGT)GTACCTTGTCCTTGTATTTATTTTTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605225637_53ew AGACTTAAAAAAAAAATAAATACAAGGACAAG(+CAGTGT)GTACCCGCACAGCGGAAAGGAGATACAATGA NGG 10 8 17 8 24 0 100 5 10