Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225544_6319 TCAATATCAGGCTTGAGATTTGTTAGTGGTCG(+CAGTGT)GATCCACGAGCTATGGCTGATGTAGAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605225543_shc7 TGGCTCATCCTCTACATCAGCCATAGCTCGTG(+CAGTGT)GATCCGACCACTAACAAATCTCAAGCCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605225540_gh6m ACCCCATGCTGCTCTCAAAAGGCTAAACCCTG(+CAGTGT)GTACCCGTGGTGGCCCTTGCTCCAGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605225538_7fe9 GCCTCCCTGCACTGGAGCAAGGGCCACCACGG(+CAGTGT)GTACCAGGGTTTAGCCTTTTGAGAGCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605225537_j5z8 TTCCCACCAAGCTAGGGCGTGGCTAGATAAAG(+CAGTGT)GTACCTCTGACTTGGGTTTCTAACAGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605225535_8tpj ATGTATCACGCTGTTAGAAACCCAAGTCAGAG(+CAGTGT)GTACCTTTATCTAGCCACGCCCTAGCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605225534_hsnm AGTTTAAACCTACATACCCAGTTTCCTTTCAG(+CAGTGT)GTACCTTGACCTGCTTCCAGGGGAACTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605225533_xix5 GCTATTAAAAGTTCCCCTGGAAGCAGGTCAAG(+CAGTGT)GTACCTGAAAGGAAACTGGGTATGTAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605225531_vs3p TTCCAAGGATGGAACTCAGGTGGTTAGTATTG(+CAGTGT)GTACCAGGAACCTAAACTTACCCTTTGAACC NGG 10 8 17 8 24 0 100 5 10
    240605225529_2cjg GGGTTGGTTCAAAGGGTAAGTTTAGGTTCCTG(+CAGTGT)GTACCAATACTAACCACCTGAGTTCCATCCT NGG 10 8 17 8 24 0 100 5 10