Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225439_u6dp ATACTCAAGTCTCCTATTTGTTCAACCAAGGG(+CAGTGT)GATCCAATAAGATGTACTAGCGAGTATTTAT NGG 10 8 17 8 24 0 100 5 10
    240605225437_fdqt TGCTCATAAATACTCGCTAGTACATCTTATTG(+CAGTGT)GATCCCCTTGGTTGAACAAATAGGAGACTTG NGG 10 8 17 8 24 0 100 5 10
    240605225436_hxct CCAAATATTTATATTTACATACATTTCAAAGG(+CAGTGT)GATCCAGTGATTGATATAAGATTCAGTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605225433_35xh CCATGTTAAACTGAATCTTATATCAATCACTG(+CAGTGT)GATCCCTTTGAAATGTATGTAAATATAAATA NGG 10 8 17 8 24 0 100 5 10
    240605225431_8cq6 AATGCCTGCAAACATTCAGACTAATCAACGTG(+CAGTGT)GTACCGTAATTTCTTTTAATACATCAATAAT NGG 10 8 17 8 24 0 100 5 10
    240605225430_bz19 AAACTATTATTGATGTATTAAAAGAAATTACG(+CAGTGT)GTACCACGTTGATTAGTCTGAATGTTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605225428_8uiw CACACTTGCCTGGCGTGTAAGGCACTGGGTTG(+CAGTGT)GATCCTCAGTACTTTGAAGAAAAGCAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605225426_e56y TCAGGGCTTTTGCTTTTCTTCAAAGTACTGAG(+CAGTGT)GATCCAACCCAGTGCCTTACACGCCAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605225425_4c1g ACTGTCACGCTGCACACGTTTTACTGCTTGAG(+CAGTGT)GATCCTACATCTGTGTTTTAGGTCTTTGATG NGG 10 8 17 8 24 0 100 5 10
    240605225424_5rxs GGTCACATCAAAGACCTAAAACACAGATGTAG(+CAGTGT)GATCCTCAAGCAGTAAAACGTGTGCAGCGTG NGG 10 8 17 8 24 0 100 5 10