Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225332_81hu CAAGGGTAAGGAACCATTAGCTGTGCCTTGGG(+CAGTGT)GATCCTTAGGGCCGGAGTAGGGCCACCCCAA NGG 10 8 17 8 24 0 100 5 10
    240605225330_6aqc ATAAGAGCATCCCTGCCCTGCCTGAGTCAGGG(+CAGTGT)GATCCACAGCCCCATTGGGACAAGAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240605225328_d0mx GGTCCCTGCATTCTTGTCCCAATGGGGCTGTG(+CAGTGT)GATCCCCTGACTCAGGCAGGGCAGGGATGCT NGG 10 8 17 8 24 0 100 5 10
    240605225327_owd9 GACCCCCAGCACCTTGGCCCACCAGAGGCTGG(+CAGTGT)GTACCCAGGCAAGGGATGTTTCAAGGCACAT NGG 10 8 17 8 24 0 100 5 10
    240605225325_i2qm ATTGTATGTGCCTTGAAACATCCCTTGCCTGG(+CAGTGT)GTACCCAGCCTCTGGTGGGCCAAGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605225323_t0hs ACTGTGGGTGCTACACACAAACACTTGGGCAG(+CAGTGT)GTACCCTACTGTCCTATAACTTCCCTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605225322_xcwn GTCTAAAGGCAGGGAAGTTATAGGACAGTAGG(+CAGTGT)GTACCTGCCCAAGTGTTTGTGTGTAGCACCC NGG 10 8 17 8 24 0 100 5 10
    240605225320_87n3 GCCAGCCAGTAATGACCACATCTCTCCATAGG(+CAGTGT)GATCCATGGGGAAGCTGGTGTTCGTCGGATA NGG 10 8 17 8 24 0 100 5 10
    240605225318_q1qo ACCTTTATCCGACGAACACCAGCTTCCCCATG(+CAGTGT)GATCCCTATGGAGAGATGTGGTCATTACTGG NGG 10 8 17 8 24 0 100 5 10
    240605225316_wusy AGGACTTTCAGATGGATTGATGAATCTTGCTG(+CAGTGT)GTACCAGTTTCACTACCAAAAGGTTGTCAAA NGG 10 8 17 8 24 0 100 5 10